Microbial ATP Bioluminescence rapid testing of Pet Food 14

Based on initial testing with canned pet food samples - sterile as well as contaminated ones - a Microbial ATP Detection Test Kit for pet food products was developed by Promicol. Together with a specific protocol this reagent setup can be used for analysis of canned pet food.

For testing, the pre-incubated samples need to be pipetted into a microplate well and the Promilite M4 instrument performs the assay automatically by using the implemented protocol which is described below:

First, samples are treated with specific reagents to remove all sources of non-microbial ATP from the samples. This step is crucial to ensure highest sensitivity of the assay. Afterwards a microbial extractant is added in order to release any microbial ATP from microorganisms present in the sample. In the end the firefly enzyme is used to turn the released microbial ATP into light. This is measured by the Promilite M4 instrument and expressed in Relative Light Units (RLU). The software automatically classifies the measured values as pass, re-test or fail and therefore enables an easy interpretation for the user.

Promicol aims to develop a long term relationship with its customers, built on trust, innovation and expertise. It is our goal to provide a service that is in touch with the needs of your quality management and to your demands from rapidly changing markets, making ATP work for you, together with you.

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