Microbial ATP Bioluminescence rapid testing of Soya Sauce 04
Based on initial testing it turned out that a specific product and sample preparation is required to perform sterility testing of Soya Sauce. Afterwards the Microbial ATP Detection Test Kit for Soya Sauce was developed. This reagent setup can be used together with a specific protocol and one of Promicols instruments for microbial ATP detection in Soya Sauce.
For testing, the pre-incubated samples need to be pipetted into a microplate well and the Promilite M4 instrument performs the assay automatically by using the implemented protocol which is described below:
First, samples are treated with specific reagents to remove all sources of non-microbial ATP from the samples. This step is crucial to ensure highest sensitivity of the assay. Afterwards a microbial extractant is added in order to release any microbial ATP from microorganisms present in the sample. In the end the firefly enzyme is used to turn the released microbial ATP into light. This is measured by the Promilite M4 instrument and expressed in Relative Light Units (RLU). The software automatically classifies the measured values as pass, re-test or fail and therefore enables an easy interpretation for the user.
Even very low levels of inoculum of specific microorganisms were identified as positive using the Promicol method.
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